The transcriptome of the baculovirus Autographa californica Multiple 1 Nucleopolyhedrovirus ( AcMNPV ) in Trichoplusia ni cells
نویسندگان
چکیده
The transcriptome of the baculovirus Autographa californica Multiple 1 Nucleopolyhedrovirus (AcMNPV) in Trichoplusia ni cells 2 3 4 Yun-Ru, Chen, Silin Zhong, Zhangjun Fei, Yoshifumi Hashimoto, Jenny Z. Xiang, 5 Shiying Zhang, and Gary W. Blissard 6 7 8 Boyce Thompson Institute at Cornell University, Tower Road, Ithaca NY 14853 9 Current address: Protein Sciences Corporation, 1000 Research Parkway, Meriden, CT 10 06450, USA 11 Weill Medical College, Cornell University, New York, NY 10021, USA 12 13 *Corresponding author 14 Gary W. Blissard 15 Boyce Thompson Institute at Cornell University 16 Tower Road, Ithaca, NY 17 Phone: 607-254-1366 18 Email: [email protected] 19 20 21 Running Title: Baculovirus AcMNPV transcriptome in T. ni cells 22
منابع مشابه
The Trichoplusia ni granulovirus helicase is unable to support replication of Autographa californica multicapsid nucleopolyhedrovirus in cells and larvae of T. ni.
Baculovirus DNA helicases are essential for replication and are determinants of host range. Helicases of Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) and Trichoplusia ni granulovirus (TnGV) differ markedly, although both viruses replicate efficiently in the cabbage looper, T. ni. To determine whether the TnGV helicase (P137) could support replication of AcMNPV in T. ni cells...
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To determine the genetic diversity within the baculovirus species Autographa calfornica multiple nucleopolyhedrovirus (AcMNPV; Baculoviridae: Alphabaculovirus), a PCR-based method was used to identify and classify baculoviruses found in virus samples from the lepidopteran host species A. californica, Autographa gamma, Trichoplusia ni, Rachiplusia ou, Anagrapha falcifera, Galleria mellonella, an...
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UNLABELLED Productive infection of Trichoplusia ni cells by the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) leads to expression of ~156 viral genes and results in dramatic cell remodeling. How the cell transcriptome responds to viral infection was unknown due to the lack of a reference genome and transcriptome for T. ni. We used an ~60-Gb RNA sequencing (RNA-seq) d...
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Enhancin genes have been identified in a number of baculoviruses and enhancin proteins are characterized by their ability to enhance the oral infectivity of heterologous baculoviruses in various lepidopteran insects. Here, we describe the putative enhancin gene from Mamestra configurata nucleopolyhedrovirus (MacoNPV), only the second NPV in which an enhancin-like ORF has been identified. The pu...
متن کاملThe transcriptome of the baculovirus Autographa californica multiple nucleopolyhedrovirus in Trichoplusia ni cells.
Baculoviruses are important insect pathogens that have been developed as protein expression vectors in insect cells and as transduction vectors for mammalian cells. They have large double-stranded DNA genomes containing approximately 156 tightly spaced genes, and they present significant challenges for transcriptome analysis. In this study, we report the first comprehensive analysis of AcMNPV t...
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